Journal: bioRxiv

Article Title: Microsecond pulse electrical stimulation modulates cell migration

doi: 10.1101/2022.10.23.513372

Figure Lengend Snippet: (a) A graphical illustration showed the effects of μsPEF (pulse width:20 μs, frequency: 10 Hz, duration: 5 s) on the skin wound, promoting cell migration and extracellular matrix remodeling. (b) Representative time-lapse images showing the fibroblasts morphology after μsPEF (i.e., 750 and 1500 V/cm) treatment of different intensity at different time points (i.e., 0, 1 and 2 h). Detached cells are marked by yellow arrow. Scale bar, 50 μm. (c) The line graph representing the cell migration average speed per 1 h from 0 to 12 h after different intensity μsPEF (i.e., 750 and 1500 V/cm) treatment (blue circle: control; orange square: 750 V/cm; pink triangle: 1500 V/cm). Results are presented as mean ± standard deviation with 95% CI (n CTRL =42 cells, n 750 v/cm =48 cells, n 1500 v/cm =47 cells); * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons. (d) Box plot showing the average cell migration speed with different intensity μsPEF (i.e., 750 and 1500 V/cm) and control treatments in 24 h. Results are presented as mean ± standard deviation with 95% CI (n CTRL =470 cells, n 750 V/cm =979 cells, n 1500 V/cm =535 cells). (e) Effects of μsPEF on the secretion of COLA2. The level of collagen type I α2 in cellular supernatants was measured after 48 h. The concentration of COLA2 was 0.109 ± 0.018 ng/mL in the control group, 0.257 ± 0.058 ng/mL in the 750 V/cm group and 0.363 ± 0.034 ng/mL in the 1500 V/cm group. Results are presented as mean ± standard deviation with 95% CI (n=5); * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons. (f) Effects of μsPEF on the expression of FGF2. The level of FGF2 in cellular supernatants was measured after 48 h. The concentration of FGF2 was 44.139 ± 0.360 ng/mL in the control group, 48.012 ± 1.488 ng/mL in the 750 V/cm group and 48.523 ± 1.944 ng/mL in the 1500 V/cm group. Results are presented as mean ± standard deviation with 95% CI (n=3); ns=0.7329, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001 versus control by one-way ANOVA for multiple comparisons.

Article Snippet: Then, the cells were cultured in serum-free medium for 48 h. The content of type I α collagen and basic fibroblast growth factor (FGF-2) in the supernatant were measured using commercially available Human COL1A2 (Collagen Type I Alpha 2) ELISA Kit (Elabscience, Wuhan, China) and Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit (Elabscience, Wuhan, China) according to the manufacturer’s protocol respectively.

Techniques: Migration, Control, Standard Deviation, Concentration Assay, Expressing

Journal: Molecular medicine reports

Article Title: β‑ecdysterone protects against apoptosis by promoting autophagy in nucleus pulposus cells and ameliorates disc degeneration.

doi: 10.3892/mmr.2019.9861

Figure Lengend Snippet: Figure 6. β‑ecdysterone regulates the expression of degeneration‑associated genes via autophagy. (A) The mRNA expression of Col2a1, Aggrecan, Adamts‑5 and MMP‑3 were measured by reverse transcription quantitative polymerase chain reaction in the nucleus pulposus cells from normal human intervertebral discs. (B) The protein expression levels of Col2a1, Aggrecan, Adamts‑5 and MMP‑3 were measured by ELISA in the nucleus pulposus cells from normal human intervertebral discs. (C) The protein expression levels of Col2a1, Aggrecan, Adamts‑5 and MMP‑3 were measured by ELISA in the nucleus pulposus cells from patients with IDD. (D) The protein levels of LC3, beclin‑1 and p62 were determined by western blot analysis in nucleus pulposus cells from patients with IDD. Cell viability and apoptosis were determined by (E) CCK8 and (F) western blot analysis assays in nucleus pulposus cells from patients with IDD. *P<0.05 and **P<0.01 vs. control. Col2a1, collagen type II alpha 1; Adamts‑5, a disintegrin and metalloproteinase with thrombospondin motifs 5; MMP‑3, matrix metalloproteinase 3; IDD, intervertebral disc degeneration; LC3, Microtubule‑associated proteins 1A/1B light chain 3A; p62, sequestosome‑1.

Article Snippet: The concentration of Col2a1 (cat. no. DY7589-05), Aggrecan (cat. no. DY1220), Adamts-5 (DY2198-05) and MMP-3 (cat. no. DMP300) in cell culture supernatants was measured using ELISA kits (R&D Systems Inc., Minneapolis, MN, USA) according to the manufacturer's protocol.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: International journal of cosmetic science

Article Title: Moisturizing and antioxidant factors of skin barrier restoring cream with shea butter, silkflo and vitamin E in human keratinocyte cells.

doi: 10.1111/ics.13014

Figure Lengend Snippet: FIGURE 5 Effect of MZ on procollagen type 1 level. Values are expressed as average of triplicate experiment and are represented as mean ± SEM. * represent significant difference from H2O2 induced group (p < 0.05), # represent significant difference from untreated cells.

Article Snippet: Estimation of type I procollagen The level of type I procollagen was measured using a human type I procollagen ELISA kit (Elabscience Biotechnology, USA).

Techniques: